Intermediate

Part:BBa_K2172008:Design

Designed by: Bowen Xiao   Group: iGEM16_CIEI-BJ   (2016-10-14)


GST-Thrombin Protease-SmCPS1-TEV-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2580
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1341
    Illegal BamHI site found at 673
    Illegal BamHI site found at 939
    Illegal XhoI site found at 1075
    Illegal XhoI site found at 1099
    Illegal XhoI site found at 2392
    Illegal XhoI site found at 2602
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 744
    Illegal NgoMIV site found at 2161
    Illegal NgoMIV site found at 2725
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


Design Notes

Two extra sticky ends are added to the gene when it is cloned via PCR. They are needed so that the part can be loaded onto other vectors. Site-directed mutagenesis is performed to alter the triplets identical to the sequence of restriction enzymes used to remove the part from the vector.


Source

SmCPS1 comes from the genome of Salvia miltiorrhiza.


References